Search results for "Bovine Pituitary Extract"

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2018

Purpose To establish a simplified three-dimensional (3D) meibomian gland culture model using a meibomian gland epithelial cell (HMGEC) line that might be a useful tool to gain deeper insights into meibomian gland dysfunction. For this purpose, 3D differentiation patterns and growth characteristics of HMGECs were studied on various membranes/scaffolds as well as in hanging drops. Methods Several types of inserts consisting of different materials (Millicell-HA, Millicell-PCF, ThinCert, and Alvetex) as well as hanging drop culture were analyzed. Culture conditions were optimized employing exposure to air (air-lift) and different cell culture media for a maximum of 28 days. To characterize cell…

0301 basic medicinechemistry.chemical_classificationChemistryCellular differentiationMeibomian glandCell biology03 medical and health sciences030104 developmental biology0302 clinical medicinemedicine.anatomical_structureEpidermal growth factorCell cultureKeratin030221 ophthalmology & optometryUltrastructuremedicineBovine Pituitary ExtractKeratinocyteInvestigative Opthalmology & Visual Science
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